Counting Single Dna Molecule by On-bead Rolling Circle Amplification for Quantitative Analyses
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چکیده
To realize an ultra sensitive determination of specific DNA, we developed and optimized on-bead rolling circle amplification (RCA) method for quantitative analyses. By using agarose beads, detection efficiency increased 100 times compared to the conventional RCA method, and quantitative analyses of 1 μL of samples containing 0.05 – 1.0 nM DNA were realized.
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تاریخ انتشار 2011